2020 – Atual – Observatório de teoria da mudança em resiliência a desastres

Projeto certificado pelo(a) coordenador(a) Adelaide Cassia Nardocci em 25/01/2021.

Situação: Em andamento; Natureza: Pesquisa.

Integrantes: Maria Tereza Pepe Razzolini – Integrante / Adelaide Cássia Nardocci – Coordenador / Mathew Kurian – Integrante / Eduardo Mario Mediondo – Integrante.

2020 – Atual – Multicentre evaluation of the Impact of COVID-19 pandemic on 4 the spread of antimicrobial and biocide resistance from wastewater to the environment

Descrição: Coronavirus disease 2019 caused by a virus known as Severe Acute Respiratory Syndrome Coronavirus , arose at the time of great concern about antimicrobial resistance (AMR), one of the actual major health challenge worldwide. Hygiene measures to prevent COVID-19 contamination, coupled to the use of antimicrobials for curative and preventive purposes could have an impact on the evolution of AMR. This multicentric study to be carried out in Brazil, Cameroon and Madagascar aims at evaluating the impact of the COVID-19 pandemic on the spread of antimicrobials and biocides resistance in the environment, especially from sewage of healthcare facilities receiving COVID-19 patients, municipal wastewaters and slum. Culture and Whole genome sequencing will enable evaluation of bacteria (diversity and relative abundance) and antimicrobial/biocide resistance genes respectively. Phylogenetic relationships between the isolated strains will be investigated through MLST and single nucleotide polymorphisms. In order to understand the link between epidemiology of COVID-19 and diversity of resistance factors, detection and quantification of SARV-CoV2 will be done by RT-qPCR. The physicochemical parameters of the study sites 43 will be analysed and their abundance in antimicrobial and biocide residues will be determined using Liquid Chromatography coupled with Mass Spectrometry. This study will provide timely information on the potential risk of spread of AMR in the downstream ecology during the Covid-19 pandemic and its public health consequences.

Situação: Em andamento; Natureza: Pesquisa.

Integrantes: Maria Tereza Pepe Razzolini – Coordenador / Nzouankeu Ariane – Integrante / Samison Luc Hervé – Integrante / Silvia Figueiredo Costa – Integrante.

2020 – Atual – Wastewater surveillance: from COVID-19 to long-term vision

Descrição: The COVID-19 pandemic has, once again, highlighted the importance of access to safe water and sanitation (SDG6). Molecular markers for microbial pathogens, including SARS-CoV-2, can be detected in wastewaters of infected communities, during an outbreak and sometimes even before the first cases are confirmed. Community surveillance through wastewater monitoring (by RT-qPCR of virus markers) is an effective tool to assist in tracking disease prevalence and guiding public health responses. Unlocking the full power of this tool to reveal the wastewater surveillance ?signal? requires additional modelling to remove the ?noise? of confounding factors (e.g. sewage dilution by rainfall, the effect of pH and temperature). This CATALYST Work Package is a rare opportunity to share knowledge and trial modified approaches at the forefront of a very rapidly evolving field that will influence active monitoring programmes in both Brazil and Wales. Building on complementary expertise in environmental surveillance, and two years of networking between the water communities of CU and UNICAMP, this WP will investigate the challenges, opportunities and alternatives for wastewater surveillance in Brazil.

Situação: Em andamento; Natureza: Pesquisa.

Integrantes: Maria Tereza Pepe Razzolini – Coordenador / Maria Ines Zanoli Sato – Integrante / Adelaide Cássia Nardocci – Integrante / Marcello Lauretto – Integrante / Isabelle Durance – Integrante / Andy Weightman – Integrante / Owen Jones – Integrante / Michaela Bray – Integrante / Henry Wild – Integrante / Flavia Uchoa – Integrante / Pete Kille – Integrante / Devin Sapsford – Integrante / Cassiana C. Montagner – Integrante.

2020 – Atual – OCORRÊNCIA DE ESTAFILOCOCOS NA ÁGUA DE BEBEDOUROS EM TERMINAIS DE ÔNIBUS DA CIDADE DE SÃO PAULO, BRASIL.

Descrição: Monitoramento de microrganismos patogênicos em água destinada ao consumo humano em dispositivos de acesso público. Identificar e quantificar características de resistência e virulência, assim como o comportamento da comunidade microbiana patogênica sobrevivendo nesse ambiente, afim de avaliar o riscos á saúde associados..

Situação: Em andamento; Natureza: Pesquisa.

Alunos envolvidos: Doutorado: (1) .

Integrantes: Geyse Aparecida Cardoso dos Santos – Francisca Alzira dos Santos Perternella – Miriam Lopes da Silva (Integrantes) / Maria Tereza Pepe Razzolini – Coordenadora.

2019 – One Health Approach to Address the Global Burden of Antimicrobial Resistance

O projeto faz parte do consórcio entre quatro universidades – USP, Unioversity of North Carolina, University of Surrey and University of Wollongong denominado de University Global Partnership Network (UGPN) o qual financia projetos liderados por pesquisadores das quatro universidades.

Integrantes: Maria Tereza Pepe Razzolini – Coordenador / Sid Thakur – Integrante / Johnjoe McFadder – Integrante / Antoine van Oijen – Integrante / Mitchell Byrne – Integrante.